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If your questions pertains to software for Nintendo consoles then the answer is yes. As the previous poster stated, there is a switch in the base that can be moved to indicate that it is an amiibo or just a Skylanders' figurine.
If your questions pertains to using the Donkey Kong or Bowser figurines as Skylanders' figurines in the game for the Xbox 360/Xbox one or the PS3/PS4. Then the answer is no. They only work with the Skylanders' software for the Nintendo systems.